The design of biosensors is one of the most important areas of analytical chemistry today, and interest in DNA-based bio (nano) sensors developed to examine applications related to compound-DNA interaction has been increasing in the last two decades. Changing in DNA structure, even for therapeutic purposes, can have serious effects on human health. The detection of any chemical substances in DNA sutructure is very important. In this study, an anticancer drug Tamoxifen (TAM) is used in the treatment of cancer since the early 1970s was identifying the possible DNA interaction during treatment by using differential pulse voltammetry (DPV) based on both TAM and guanine oxidation signals at the disposable pencil graphite electrode (PGE). The effect of TAM on single stranded (ss)-DNA and double stranded (ds)-DNA showed differences, depending on the double helix and single stranded structure. It was found that TAM interacting to ds-DNA more strongly than ss-DNA. Thus, Drug-DNA interaction analysis has been investigated for the first time under optimized conditions with the Tamoxifen which, gave an oxidation peak potential near the guanine oxidation area. These results presented that the developed DNA biosensor could be detected TAM-DNA interaction as a sensitive, rapid and cost effective way. Electrochemical detectionTamoxifen recovery from commercial tablets was also studied.
CAN S., YILMAZ S., SAGLIKOGLU G., SADIKOGLU M., MENEK N., 2015, Electrocatalytic oxidation of acyclovir on poly (p-aminobenzene sulfonic acid) film modified glassy carbon electrode, Electroanalysis, 27, 2431.
HURTADO-MONROY R., VARGAS-VIVEROS P., CARRILLO-MUÑNOZ S., DUENAS-GONZALEZ A., 2007, Tamoxifen-associated vasculitis in a breast cancer patient, World Journal of Surgical Oncology, 5-7.
JAHANDARI S., TAHER M. A., KARIMI-MALEH H., KHODADADI A., FAGHIH-MRZAEI E., 2019. A Powerful DNA-based voltammetric biosensor modified with au nanoparticles, for the determination of temodal; an electrochemical and docking investigation. Journal of Electroanalytical Chemistry, 840, 313-318.
KELSEY J. L., BERNSTEIN L., 1996, Epidemiology and Prevention of Breast Cancer, Annual Review of Public Health, 17:47-67.
MOGHADDAM H. M., BEITOLLAHI H., DEHGHANNOUDEH G., FOROOTANFARD H., 2017, The electrochemical society a label-free electrochemical biosensor based on carbon paste electrode modified with graphene and ds-dna for the determination of the anti-cancer drug tamoxifen, Journal of The Electrochemical Society, 164 (7) 372-376.
OSBORNE C. K.,1998, Tamoxifen in the treatment of breast cancer, N Engl J Med, 339:1609-1618.
OZKAN D., KARADENIZ H., ERDEM A., MASCINI M., OZSOZ M., 2004, Electrochemical genosensor for mitomycin c- dna interaction based on guanine signal, Journal of Pharmaceutical and Biomedical Analysis, 35(4): 905-912.
R. D. SYNDER, J. E. BROWN, 2002, Evidence for and role of the dimethylamino group in tamoxifen DNA intercalation in intact chinese hamster v79 cells, Drug Chem Toxicol., 25(4):473-9.
SADIKOGLU M., YILMAZ S., KURT I., SELVI B., SARI H., ERDURAN N., USTA E., SAGLIKOGLU G., 2016, Electrocatalytic oxidation of hydrazine on poly (4 aminobenzene sulfonic acid)-modified glassy carbon electrode, Russian J. Electrochem. 52, 603.
SUBAK H., OZKAN-ARIKSOYSAL D., 2018. Label-free electrochemical biosensor for the detection of ınfluenza genes and the solution of guanine-based displaying problem of dna hybridization. Sens. Actuators B, 263, pp. 196-207.
TEUNISSENS.F., ROSING H., SCHINKELA.H., SCHELLENSJ.H.M., BEIJNENJ.H.,2010, Bioanalytical methods for determination of tamoxifen and its phase I metabolites: a review, Analytica Chimica Acta, 683, 21-37.
GUPTA V.K., JAIN R., RADHAPYAII K., JADON N., AGARWAL S., 2011, Voltammetric techniques for the assay of pharmaceuticals-a review, Anal. Biochem., 408, 79-196.
YANG Q., BENSON L. M., JOHNSONK.L., NAYLORS., 1999, Analysis of lipophilic peptides and therapeutic drugs: on-line-nonaqueous capillary electrophoresis-mass spectrometry.J. Biochem. Biophys. Methods, 38, 103-121.