ELECTROCHEMICAL BIOSENSOR FOR BRCA1 GENE AND TAMOXIFEN INTERACTION
Authors
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Suzan YANIK
Çanakkale Onsekiz Mart University / TURKEY
0000-0002-0532-4149
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Dilşat ÖZKAN-ARIKSOYSAL
University of Ege / TURKEY
0000-0002-8471-5665
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Selehattin YILMAZ
Çanakkale Onsekiz Mart University / TURKEY
0000-0003-4607-3523
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18198
Abstract
The electrochemical nanobiosensor was designed for the determination of specific DNA sequences related to breast cancer 1 (BRCA1) gene and interaction between Anticancer Drug Tamoxifen (TAM) and related DNA sequences by using pencil graphite electrode (PGE), bare and multi-walled carbon nanotube (MWCNT) contained screen printed carbon electrodes (SPEs) for the first time. Here, biomolecular interaction between TAM and DNA was investigated differential pulse voltammetry (DPV) based on not only guanine signal but also TAM oxidation response. It was obtained that the guanine signal at about +1.00V obtained from probe DNA or hybrid DNA shows a remerkable increase after the interaction with TAM. Additionally, it was found that TAM interact with guanine bases and TAM signal which is near the guanine oxidation area also increase after the interaction with DNA. Consequently, the prepared biosensor offer suitable platform for the analysis of DNA hybridization and TAM-DNA interaction sensitively.
Keywords:
Tamoxifen BRCA1 Electrochemical DNA nanobiosensor carbon nanotube Differential Pulse Voltammetry Drug-DNA interaction Pencil Graphite Electrode Screen Printed ElectrodeDownloads
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