VOLTAMMETRIC DETERMINATIN OF CLOZAPINE FROM ITS DRUG FORM

Abstract

A voltammetry method was developed for the direct quantitative determination of clozapine in tablet dosage forms based on its oxidation behavior.  The electrochemical determination of clozapine was easily carried out on glassy carbon electrode (GCE) using a variety of voltammetry techniques.  The electrochemical measurements were carried out on GCE in various buffer solutions in the pH range from 3.00 to 12.00 by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques.  The dependence of pH on the anodic peak current and peak potential was investigated. Acetate buffer (pH 5.50) was selected for analytical purposes.  The diffusion-controlled nature of the peak was established.   A linear calibration curve for DPV analysis was constructed in the clozapine concentration range from 3x10^-6 mol L^-1 to 1x10^-5 mol L^-1. Limit of detection (LOD) and limit of quantification (LOQ) were obtained as 4.082x10^-7 mol L^-1 and 1.361x10^-6 mol L^-1 respectively.  Validation of applied voltammetric techniques was checked with recovery studies.