DETERMINATION OF CLOMIPRAMINE HYDROCHLORIDE FROM ITS COMMERCIAL DRUG FORM BY VOLTAMMETRY

Abstract

In this study, electroanalytical technique was developed for the quantitative analysis of clomipramine hydrochloride from its commercial tablet dosage forms based on its oxidation behavior.  The electrochemical determination of clomipramine hydrochloride was easily carried out on glassy carbon electrode (GCE) by two voltammetric techniques.  The electrochemical measurements were carried out on GCE surface in different buffer solutions in the pH range from 2.00 to 12.00 by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques.  The efect of pH on the anodic peak current and peak potential was investigated. Phosphate buffer (pH 6.50) was selected for analytical purposes.  The diffusion-controlled nature of the peak was obtained.   A linear calibration plot for DPV analysis was constructed in the clozapine concentration range from 5x10^-6 mol L^-1 to 3x10^-5 mol L^-1. Limit of detection (LOD) and limit of quantification (LOQ) were obtained as 2.8x10^-7 mol L^-1 and 8.4x10^-7 mol L^-1 respectively.  Validation of applied voltammetric techniques was applied.