VOLTAMMETRIC ANALYSIS OF TAMOXIFEN RECOVERY AND ITS INTERACTION WITH DNA

Abstract

The design of biosensors is one of the most important areas of analytical chemistry today, and interest in DNA-based bio (nano) sensors developed to examine applications related to compound-DNA interaction has been increasing in the last two decades. Changing in DNA structure, even for therapeutic purposes, can have serious effects on human health. The detection of any chemical substances in DNA sutructure is very important. In this study, an anticancer drug Tamoxifen (TAM) is used in the treatment of cancer since the early 1970s was identifying the possible DNA interaction during treatment by using differential pulse voltammetry (DPV) based on both TAM and guanine oxidation signals at the disposable pencil graphite electrode (PGE). The effect of TAM on single stranded (ss)-DNA and double stranded (ds)-DNA showed differences, depending on the double helix and single stranded structure. It was found that TAM interacting to ds-DNA more strongly than ss-DNA. Thus, Drug-DNA interaction analysis has been investigated for the first time under optimized conditions with the Tamoxifen which, gave an oxidation peak potential near the guanine oxidation area. These results presented that the developed DNA biosensor could be detected TAM-DNA interaction as a sensitive, rapid and cost effective way. Electrochemical detectionTamoxifen recovery from commercial tablets was also studied.