EFFECTS OF SOME OF HEAVY METALS ON TOTAL PROTEIN AMOUNT AND PEROXIDASE ACTIVITY IN SOLANUM LYCOPERSICUM MILL
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https://doi.org/10.26900/jsp.3.037Keywords:
Solanum lycopersicum, cadmium, nickel,, copper, total protein, peroxidaseAbstract
In this research, different concentrations of cadmium, nickel, copper heavy metals applied on 10 weeks old Solanum lycopersicum Mill. cv. invictus seedlings by irrigation water. As a result of heavy metal applications total protein amount and peroxidase [EC 1.11.1.7] enzyme activity has been determined by spectrophotometrically. Changing in total protein amount and peroxidase activity in S. lycopersicum seedlings after heavy metal applications has been compared with control group. Depending the application of heavy metals on S. lycopersicum seedlings total protein amount decreased as 57% in 100 ppm of copper application and 10,9% increased in 10 ppm nickel application. The highest increases in peroxidase activity 100 ppm of copper applied and in proportion as 536,03% and treated group with 10 ppm nickel in proportion as 5.97%were determined.
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References
BALESTRASSE, K.B., BENAVIDES MP, GALLEGO SM, TOMARO M.L., 2003, Effect of Cadmium Stress on Nitrogen Metabolism in Nodules and Roots of Soybean Plants. Functional Plant Biology, 30: 57-64.
https://www.researchgate.net/publication/248887499_Effect_of_cadmium_stress_on_nitrogen_metabolism_in_nodules_and_roots_of_soybean_plants
BAVI K., KOHELDEBARIN B., MOREDSHAHI A., 2011, Effect of Cadmium On Growth, Protein Content And Peroxidase Activity, In Pea Plants. Pak. J. Bot., 43 (3), 1467-1470.
http://www.pakbs.org/pjbot/PDFs/43(3)/PJB43(3)1467.pdf
BHALERAO S.A., SHARMA A.S., POOJARI A.C., 2015, Toxicity of Nickel in Plants. International Journal Of Pure & Applied Bioscience. 3 (2), 345-355.
http://www.ijpab.com/form/2015%20Volume%203,%20issue%202/IJPAB-2015-3-2-345-355.pdf
BRADFORD M.M., 1976, A Rapid and Sensitive Method for the Quantitation of Microgram Quantites of Protein Utilizing the Principle of Protein-Dye Binding, Analytical Biochemistry, 72, 248-254.
http://hoffman.cm.utexas.edu/courses/bradford_assay.pdf
CHAVES L.H.G., ESTRELA M.A., SOUZA R.S., 2011, Effect on Plant Growth and Heavy Metal Accumulation by Sunflower. Journal of Phytology, 3 (12), 04-09.
https://www.researchgate.net/publication/266589410_Effect_on_plant_growth_and_heavy_metal_accumulation_by_sunflower/fulltext/54b000ce0cf2431d3531ce85/Effect-on-plant-growth-and-heavy-metal-accumulation-by-sunflower.pdf
ÇÖRDÜK N, AKINCI N, KAYA N, YÜCEL G, AKI C., 2016, Effects of Dodine on Total Protein Content and Peroxidase Activity in Vicia faba L., SAÜ Fen Bil Der 20 (3), 627-633.
http://www.saujs.sakarya.edu.tr/tr/download/article-file/236526
DALCORSO G., MANARA A., PIASENTIN S., FURINI A., 2014, Nutrient Metal Elements in Plants. Metallomics, 6 (10), 1770-88.
https://www.ncbi.nlm.nih.gov/pubmed/25144607
EMAMVERDIAN A., DING Y., MOKHBERDORAN F., XIE Y., 2015, Heavy Metal Stress and Some Mechanisms of Plant Defense Response, The Scientific World Journal, Volume 2015, 18 pages.
https://www.hindawi.com/journals/tswj/2015/756120/
GEORGIADOU E.C., KOWALSKA E., PATLA K, KULBAT K., SMOLISNKA B., LESZCZYNSKA J., FOTOPOULOS V., 2019, Influence of Heavy Metals (Ni, Cu, and Zn) on Nitro-Oxidative Stress Responses, Proteome Regulation and Allergen Production in Basil (Ocimum basilicum L.) Plants, Frontiers in Plant Science, 9,862, 2018.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6041727/
JOMOVA K., and MOROVIC M., 2009, Effect of Heavy Metal Treatment on Molecular Changes in Root Tips of Lupinus luteus L., Czech J. Food Sci., 27, 386-389.
https://www.agriculturejournals.cz/publicFiles/07959.pdf
KANNER, J., and KINSELLA, J.E., 1983, Lipid Deterioration Initiated by Phagocytic Cells in Muscle Foods: β-Carotene Destruction by a Myeloperoxidase-Hydrogen Peroxide Halide System, Journal of Agricultural and Food Chemistry, 31, 370-376.
https://pubs.acs.org/doi/abs/10.1021/jf00116a047
KISA D., The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato, 2017, Süleyman Demirel University Journal of Natural and Applied Sciences22 (1), 1-6.
https://dergipark.org.tr/tr/download/article-file/475601
KİRBAĞ ZENGIN F. and KİRBAĞ S., 2007, Effects of Copper on Chlorophyll, Proline, Protein and Abscisic Acid Level of Sunflower (Helianthus annuus L.) Seedlings. Journal of Environmental Biology, 28 (3), 561-566.
https://pdfs.semanticscholar.org/1313/160f417255fb45882bab703613bb83ec0075.pdf
MANAWADU, I.P., NILANTHI D., SENANAYAKE S.G.J.N, 2014, Callus Formation and Organogenesis of Tomato (Lycopersicon esculentum Mill. variety thilina), 2014, Tropical Agricultral Research and Extension, 17 (2), 87-94.
https://pdfs.semanticscholar.org/8b56/3e806772501288ab80e7e0e3b3db7671a152.pdf
PERIAGO M.J., GARCIA-ALONSO J., JACOB K., OLIVARES A.B., BERNAL M.J., INIESTA M.D., MARTINEZ C., ROS G., 2009, Bioactive Compounds, Folates and Antioxidant Properties of Tomatoes (Lycopersicum esculentum) During Vine Ripening, International Journal of Food Sciences and Nutrition, 60 (8), 694–708.
https://www.tandfonline.com/doi/pdf/10.3109/09637480701833457
RAI PK., 2016, Particulate Matter and Its Size Fractionation, Biomagnetic Monitoring of Particulate Matter, Chapter 1, pages 1-13.
https://www.sciencedirect.com/science/article/pii/B9780128051351000019?via%3Dihub
RAI P.K., LEE S.S., ZHANG M., TSANG Y.F., KIM K.H., 2019, Heavy Metals in Food Crops: Health Risks, Fate, Mechanisms and Management. Environment International 125, 365–385.
https://reader.elsevier.com/reader/sd/pii/S0160412018327971?token=FC910D99BDDD46D43C2D6580AD4791C0E9458350DF089D55F8F82F810A20EB969826FCF58E2473450FA394F29A87BCBF
RUCINISKA- SOBKOWIAK R., 2010, Oxidative Stress in Plants Exposed to Heavy Metals. Postepy Biochem., 56 (2),191-200.
https://www.ncbi.nlm.nih.gov/pubmed/20873114
SHARMA R.K., AGRAWAL M. 2005, Biological effects of heavy metals : An overview. Journal of Environmental Biology, 26 (2), 301-313.
https://www.researchgate.net/publication/7435890_Biological_effects_of_heavy_metals_An_overview
SINGH K. and Pandey S.N., 2011, Effect of Nickel-Stresses on Uptake, Pigments and Antioxidative Responses of Water Lettuce, Pistia stratiotes L. J., Environ. Biol. 32, 391-394. https://pdfs.semanticscholar.org/2426/d5122b33417d207783072f83a15f2ac72f0f.pdf
WANG C., ZHANG S.H., WANG P.F., HOU J., ZHANG W.J., LI W., LIN Z.P., 2009, The Effect of Excess Zn on Mineral Nutrition and Antioxidative Response in Rapeseed Seedlings., Chemosphere, 75 (11), 1468–1476.
https://www.ncbi.nlm.nih.gov/pubmed/19328518
YÜCEL G., 2013, Genotoxic and Enzymatic Effects of Nickel (Ni), Cadmium (Cd), Copper (Cu) Heavy Metals on In Vivo Growth Lycopersicum esculentum Mill. cv. Invictus Varieties, Master Thesis, Canakkale Onsekiz Mart University Graduate School of Natural and Applied Sciences.
https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp
https://www.researchgate.net/publication/248887499_Effect_of_cadmium_stress_on_nitrogen_metabolism_in_nodules_and_roots_of_soybean_plants
BAVI K., KOHELDEBARIN B., MOREDSHAHI A., 2011, Effect of Cadmium On Growth, Protein Content And Peroxidase Activity, In Pea Plants. Pak. J. Bot., 43 (3), 1467-1470.
http://www.pakbs.org/pjbot/PDFs/43(3)/PJB43(3)1467.pdf
BHALERAO S.A., SHARMA A.S., POOJARI A.C., 2015, Toxicity of Nickel in Plants. International Journal Of Pure & Applied Bioscience. 3 (2), 345-355.
http://www.ijpab.com/form/2015%20Volume%203,%20issue%202/IJPAB-2015-3-2-345-355.pdf
BRADFORD M.M., 1976, A Rapid and Sensitive Method for the Quantitation of Microgram Quantites of Protein Utilizing the Principle of Protein-Dye Binding, Analytical Biochemistry, 72, 248-254.
http://hoffman.cm.utexas.edu/courses/bradford_assay.pdf
CHAVES L.H.G., ESTRELA M.A., SOUZA R.S., 2011, Effect on Plant Growth and Heavy Metal Accumulation by Sunflower. Journal of Phytology, 3 (12), 04-09.
https://www.researchgate.net/publication/266589410_Effect_on_plant_growth_and_heavy_metal_accumulation_by_sunflower/fulltext/54b000ce0cf2431d3531ce85/Effect-on-plant-growth-and-heavy-metal-accumulation-by-sunflower.pdf
ÇÖRDÜK N, AKINCI N, KAYA N, YÜCEL G, AKI C., 2016, Effects of Dodine on Total Protein Content and Peroxidase Activity in Vicia faba L., SAÜ Fen Bil Der 20 (3), 627-633.
http://www.saujs.sakarya.edu.tr/tr/download/article-file/236526
DALCORSO G., MANARA A., PIASENTIN S., FURINI A., 2014, Nutrient Metal Elements in Plants. Metallomics, 6 (10), 1770-88.
https://www.ncbi.nlm.nih.gov/pubmed/25144607
EMAMVERDIAN A., DING Y., MOKHBERDORAN F., XIE Y., 2015, Heavy Metal Stress and Some Mechanisms of Plant Defense Response, The Scientific World Journal, Volume 2015, 18 pages.
https://www.hindawi.com/journals/tswj/2015/756120/
GEORGIADOU E.C., KOWALSKA E., PATLA K, KULBAT K., SMOLISNKA B., LESZCZYNSKA J., FOTOPOULOS V., 2019, Influence of Heavy Metals (Ni, Cu, and Zn) on Nitro-Oxidative Stress Responses, Proteome Regulation and Allergen Production in Basil (Ocimum basilicum L.) Plants, Frontiers in Plant Science, 9,862, 2018.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6041727/
JOMOVA K., and MOROVIC M., 2009, Effect of Heavy Metal Treatment on Molecular Changes in Root Tips of Lupinus luteus L., Czech J. Food Sci., 27, 386-389.
https://www.agriculturejournals.cz/publicFiles/07959.pdf
KANNER, J., and KINSELLA, J.E., 1983, Lipid Deterioration Initiated by Phagocytic Cells in Muscle Foods: β-Carotene Destruction by a Myeloperoxidase-Hydrogen Peroxide Halide System, Journal of Agricultural and Food Chemistry, 31, 370-376.
https://pubs.acs.org/doi/abs/10.1021/jf00116a047
KISA D., The Responses of Antioxidant System against the Heavy Metal-Induced Stress in Tomato, 2017, Süleyman Demirel University Journal of Natural and Applied Sciences22 (1), 1-6.
https://dergipark.org.tr/tr/download/article-file/475601
KİRBAĞ ZENGIN F. and KİRBAĞ S., 2007, Effects of Copper on Chlorophyll, Proline, Protein and Abscisic Acid Level of Sunflower (Helianthus annuus L.) Seedlings. Journal of Environmental Biology, 28 (3), 561-566.
https://pdfs.semanticscholar.org/1313/160f417255fb45882bab703613bb83ec0075.pdf
MANAWADU, I.P., NILANTHI D., SENANAYAKE S.G.J.N, 2014, Callus Formation and Organogenesis of Tomato (Lycopersicon esculentum Mill. variety thilina), 2014, Tropical Agricultral Research and Extension, 17 (2), 87-94.
https://pdfs.semanticscholar.org/8b56/3e806772501288ab80e7e0e3b3db7671a152.pdf
PERIAGO M.J., GARCIA-ALONSO J., JACOB K., OLIVARES A.B., BERNAL M.J., INIESTA M.D., MARTINEZ C., ROS G., 2009, Bioactive Compounds, Folates and Antioxidant Properties of Tomatoes (Lycopersicum esculentum) During Vine Ripening, International Journal of Food Sciences and Nutrition, 60 (8), 694–708.
https://www.tandfonline.com/doi/pdf/10.3109/09637480701833457
RAI PK., 2016, Particulate Matter and Its Size Fractionation, Biomagnetic Monitoring of Particulate Matter, Chapter 1, pages 1-13.
https://www.sciencedirect.com/science/article/pii/B9780128051351000019?via%3Dihub
RAI P.K., LEE S.S., ZHANG M., TSANG Y.F., KIM K.H., 2019, Heavy Metals in Food Crops: Health Risks, Fate, Mechanisms and Management. Environment International 125, 365–385.
https://reader.elsevier.com/reader/sd/pii/S0160412018327971?token=FC910D99BDDD46D43C2D6580AD4791C0E9458350DF089D55F8F82F810A20EB969826FCF58E2473450FA394F29A87BCBF
RUCINISKA- SOBKOWIAK R., 2010, Oxidative Stress in Plants Exposed to Heavy Metals. Postepy Biochem., 56 (2),191-200.
https://www.ncbi.nlm.nih.gov/pubmed/20873114
SHARMA R.K., AGRAWAL M. 2005, Biological effects of heavy metals : An overview. Journal of Environmental Biology, 26 (2), 301-313.
https://www.researchgate.net/publication/7435890_Biological_effects_of_heavy_metals_An_overview
SINGH K. and Pandey S.N., 2011, Effect of Nickel-Stresses on Uptake, Pigments and Antioxidative Responses of Water Lettuce, Pistia stratiotes L. J., Environ. Biol. 32, 391-394. https://pdfs.semanticscholar.org/2426/d5122b33417d207783072f83a15f2ac72f0f.pdf
WANG C., ZHANG S.H., WANG P.F., HOU J., ZHANG W.J., LI W., LIN Z.P., 2009, The Effect of Excess Zn on Mineral Nutrition and Antioxidative Response in Rapeseed Seedlings., Chemosphere, 75 (11), 1468–1476.
https://www.ncbi.nlm.nih.gov/pubmed/19328518
YÜCEL G., 2013, Genotoxic and Enzymatic Effects of Nickel (Ni), Cadmium (Cd), Copper (Cu) Heavy Metals on In Vivo Growth Lycopersicum esculentum Mill. cv. Invictus Varieties, Master Thesis, Canakkale Onsekiz Mart University Graduate School of Natural and Applied Sciences.
https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp
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Published
2019-10-29
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YÜCEL, G., & AKI, C. (2019). EFFECTS OF SOME OF HEAVY METALS ON TOTAL PROTEIN AMOUNT AND PEROXIDASE ACTIVITY IN SOLANUM LYCOPERSICUM MILL. HEALTH SCIENCES QUARTERLY, 3(4), 371–378. https://doi.org/10.26900/jsp.3.037
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