Effect of labor pain on placental gene expressions


Abstract views: 93 / PDF downloads: 90

Authors

DOI:

https://doi.org/10.26900/hsq.2058

Keywords:

Labor pain, SERPINET, GDF15, ADM, NOS3, IL-6, tumor necrosis factor-α (TNF-α)

Abstract

A close relationship between labor and the placenta is known. The study of gene expression profiles describing the effects of labor on placental tissue has accelerated in recent years. This study aims to compare the expression levels of GDF15, ADM, SERPINE1, NOS3, IL 6, TNF-α genes in placental tissues discarded after vaginal deliveries with labor pain and elective cesarean deliveries before the onset of labor pain. For this purpose, placental tissues of vaginal deliveries with labor pain (n=9) and elective cesarean deliveries without labor pain (n=9) were collected immediately after births. RNA extracted from tissues (umblical cords and deciduas). Gene expression analyzes were performed by Real-time PCR method. TNF-α gene expression levels in decidua and umblical cord tissues in vaginal deliveries with labor pain were higher than in elective cesareans without labor pain, and NOS3, ADM, SERPINE1 gene expression levels were low. GDF15 and IL-6 gene expression levels were high in the umblical cord tissues and were low in the decidua in vaginal deliveries with labor pain compared to elective cesareans without labor pain. The high level of GDF15 gene expression in the vaginal deliveries with labor pain umblical cord tissues was significant (p<0.05). Changes in gene expression between different types of birth may help us to understand how labor pain affects gene expression levels. These results suggest that labor pain affects different tissues in different ways. The “birth experience” of a placenta that has experienced pain stress is absolutely different to others process. Since birth pain is a natural stress, it can be a light in determining the effects of the differences in cesarean section on the baby and the mother. Gene expression alterations may cause labor starting and progressing, or just be an result of labor.

Downloads

Download data is not yet available.

References

Smith, R. Parturition. N Engl J Med. 2007;356;271-83. doi:10.1056/NEJMra061360.

Wang TH, Lee YS, Chen ES, Kong WH, Chen LK, Hsueh DW, et al. Establishment of cDNA microarray analysis at the Genomic Medicine Research Core Laboratory (GMRCL) of Chang Gung Memorial Hospital. Chang Gung Med J. 2004;27(4):243-60.

Churchill GA. Fundamentals of experimental design for cDNA microarrays. Nat Genet. 2002;32:490-5. doi: 10.1038/ng1031.

Cui X, Churchill GA. Statistical tests for differential expression in cDNA microarray experiments. Genome Biol. 2003;4(4):210. doi: 10.1186/gb-2003-4-4-210.

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001;25(4):402-8. doi: 10.1006/meth.2001.1262.

Tsai MS, Hwang SM, Chen KD, Lee YS, Hsu LW, Chang YJ, et al. Functional network analysis on the transcriptomes of mesenchymal stem cells derived from amniotic fluid, amniotic membrane, cord blood, and bone marrow. Stem Cells. 2007;25(10):2511-23. doi: 10.1634/stemcells.2007-0023.

Wang CN, Chang SD, Peng HH, Lee YS, Chang YL, Cheng PJ, et al. Change in amniotic fluid levels of multiple antiangiogenic proteins before development of preeclampsia and intrauterine growth restriction. J Clin Endocrinol Metab. 2010;95(3):1431-41. doi: 10.1210/jc.2009-1954.

Guo L, Lobenhofer EK, Wang C, Shippy R, Harris SC, Zhang L, et al. Rat toxicogenomic study reveals analytical consistency across microarray platforms. Nat Biotechnol. 2006;24(9):1162-9. doi:10.1038/nbt1238.

Affonso DD, Mayberry LJ. Common stressors reported by a group of childbearing American women. Health Care Women Int. 1990;11(3):331-45. doi: 10.1080/07399339009515902.

Kilpatrick SJ, Laros RK. Characteristics of normal labor. Obstet Gynecol. 1989;74(1):85-7.

Cunningham FG, Grant NF, Leveno KJ, Gilstrap LC, Hauth JC, Wenstrom KD. Intrapartum assessment. Williams Obstetrics. 21st ed. New York: McGraw-Hill; 2001. 331-60 p.

Peng HH, Kao CC, Chang SD, Chao AS, Chang YL, Wang CN, et al. The effects of labor on differential gene expression in parturient women, placentas, and fetuses at term pregnancy. Kaohsiung J Med Sci. 2011(11):494-502. doi:10.1016/j.kjms.2011.06.012.13. www.genecards.org, (access 2022 December 12)

Hinson JP, Kapas S, Smith DM. Adrenomedullin, a multifunctional regulatory peptide. Endocr Rev. 2000;21(2):138-67. doi: 10.1210/edrv.21.2.0396.

Hauguel-de Mouzon S, Guerre-Millo M. The placenta cytokine network and inflammatory signals. Placenta. 2006;27(8):794-8. doi:10.1016/j.placenta.2005.08.009.

McNamara H, Johnson N. The effect of uterine contractions on fetal oxygen saturation. Br J Obstet Gynaecol. 1995;102(8):644-7. doi: 10.1111/j.1471-0528.1995.tb11403.x.

Allen CJ, Griswold AJ, Schulman CI, Sleeman D, Levi JU, Livingstone AS, et al. Global gene expression change induced by major thoracoabdominal surgery. Ann Surg. 2017;266:981-7. doi: 10.1097/SLA.0000000000001992.

Schlinzig T, Johansson S, Gunnar A, Ekström T, Norman, M. Epigenetic modulation at birth: Altered DNA-methylation in white blood cells after Caesarean section. Acta Paediatr. 2009;98:1096-9. doi: 10.1111/j.1651-2227.2009.01371.x.

Kothiyal P, Schulkers K, Liu X, Hazrati S, Vilboux T, Gomez LM, et al. Differences in maternal gene expression in cesarean section delivery compared with vaginal delivery. Sci Rep. 2020;10(1):17797.doi: 10.1038/s41598-020-74989-8.

Lee KJ, Shim SH, Kang KM, Kang JH, Park DY, Kim SH, et al. Global gene expression changes induced in the human placenta during labor. Placenta. 2010(8):698-704. doi: 10.1016/j.placenta.2010.05.006.

Gibbs RS, Romero R, Hiller SL, Eschenbach DA, Sweet RL. A review of premature birth and subclinical infection. Am J Obstet Gynecol. 1992;166:1515-28. doi: 10.1016/0002-9378(92)91628-n.

Dudley DJ. Pre-term labor: An intra-uterine inflammatory response syndrome? J Reprod Immunol. 1997;36(1-2):93-109. doi: 10.1016/s0165-0378(97)00065-x.

Gomez R, Romero R, Edwin SS, David C. Pathogenesis of preterm labor and preterm premature rupture of membranes associated with intraamniotic infection. Infect Dis Clin North Am.1997;11:135-76. doi: 10.1016/s0891-5520(05)70347-0.

Pfaffl MW, Horgan GW, Dempfle L. Relative Expression Software Tool (REST©) for group wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 2002;30(9):36. doi: 10.1093/ nar/30.9.e36.

Watson A, Mazumder A, Stewart M, Balasubraminian S. Technology for microarray analysis of gene expression. Curr Opin Biotechnol.1998;9:609-14. doi: 10.1016/s0958-1669(98)80138-9.

Cindrova-Davies T, Yung HW, Johns J, SpasicBoskovic O, Korolchuk S, Jauniaux E, et al. Oxidative stress, gene expression, and protein changes induced in the human placenta during labor. Am J Pathol. 2007;171(4):1168-79. doi: 10.2353/ajpath.2007.070528.

Sitras V, Paulssen RH, Gronaas H, Vartun A, Acharya G. Gene expression profile in laboring and non-laboring human placenta near term. Mol Hum Reprod. 2008;14(1):61-5. doi: 10.1093/molehr/gam083.

Keelan JA, Blumenstein M, Helliwell RJ, Sato TA, Marvin KW, Mitchell MD. Cytokines, prostaglandins and parturitionda review. Placenta. 2003;24(A):33-46. doi: 10.1053/plac.2002.0948.

Bowen JM, Chamley L, Keelan JA, Mitchell MD. Cytokines of the placenta and extra-placental membranes: Roles and regulation during human pregnancy and parturition. Placenta. 2002;23:257-73. doi: 10.1053/plac.2001.0782.

Bowen JM, Chamley L, Mitchell MD, Keelan JA. Cytokines of the placenta and extra-placental membranes: Biosynthesis, secretion and roles in establishment of pregnancy in women. Placenta. 2002;23:239-56. doi: 10.1053/plac.2001.0781.

Marvin KW, Keelan JA, Eykholt RL, Sato TA, Mitchell MD. Use of cDNA arrays to generate differential expression profiles for inflammatory genes in human gestational membranes delivered at term and preterm. Mol Hum Reprod. 2002;8:399-408. doi: 10.1093/molehr/8.4.399.

Samson WK. Adrenomedullin and the control of fluid and electrolyte homeostasis. Annu Rev Physiol. 1999;61:363-89. doi: 10.1146/annurev.physiol.61.1.363.

Hinson JP, Kapas S, Smith DM. Adrenomedullin, a multifunctional regulatory peptide. Endocr Rev. 2000;21(2):138-67. doi: 10.1210/edrv.21.2.0396.

Marjono AB, Brown DA, Horton KE, Wallace EM, Breit SN, Manuelpillai U. Macrophage inhibitory cytokine-1 in gestational tissues and maternal serum in normal and preeclamptic pregnancy. Placenta. 2003;24(1):100-6. doi: 10.1053/plac.2002.0881.

Downloads

Published

2023-10-13

How to Cite

Arıkan Söylemez, E. S., Arıöz, D. T., & Chkhikvadze, M. (2023). Effect of labor pain on placental gene expressions. HEALTH SCIENCES QUARTERLY, 3(4), 251–258. https://doi.org/10.26900/hsq.2058

Issue

Vol. 3 No. 4 (2023)

Section

Original Article

License

Copyright (c) 2023 Holistence Publications

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

When the  article is accepted for publication in the HSQ authors transfer all copyright in the article to the Holistence Academy Ar-Ge Yazılım Yayıncılık Eğitim Danışmanlık ve Organizasyon Ticaret Ltd. Şti.The authors reserve all proprietary right other than copyright, such as patent rights. 

Everyone who is listed as an author in this article should have made a substantial, direct, intellectual contribution to the work and should take public responsibility for it.

This paper contains works that have not previously published or not under consideration for publication in other journals.