RESEARCH ON THE COMPOSITION AND CYTOTOXIC ACTIVITY OF PINUS BRUTIA GUM

Abstract

Pinus Brutia Ten., traditionaly used as sugar regulator, antioxidant and antitumoral is consumed by chewing in Turkey. The aim of the study is to investigate the components and the effect of the gum. The base of the study depends on the way of comsumption of the resin. For in-vitro analysis, artificial salvia is used. The samples is stayed in artificial salvia at 37° C in ultrasonic bath for  different time period and then determined by U-HPLC, PDA detector. Three samples were prepared from each sample and three injections were made. Caffeic acid  was investigate in the samples. The optimum waiting period is set at 5 hours, which is considered to be a possible period since the  gum adheres to the tooth and is a hardly soluble substance. Considering the anticarcinogenic effect of caffeic acid, cytotoxic test was carried out on specimens which were kept in saliva for 24 hours acid. After incubation of the saliva extract that including 10, 20, 50 µM caffeic acid  in liver cancerous cells 1, 3, 6 hours, cell viability was observed.