RESEARCH ON THE COMPOSITION AND CYTOTOXIC ACTIVITY OF PINUS BRUTIA GUM
Abstract views: 205 / PDF downloads: 186
Keywords:Pinus brutia gum, salvia, UPLC, cytotoxic activity
Pinus Brutia Ten., traditionaly used as sugar regulator, antioxidant and antitumoral is consumed by chewing in Turkey. The aim of the study is to investigate the components and the effect of the gum. The base of the study depends on the way of comsumption of the resin. For in-vitro analysis, artificial salvia is used. The samples is stayed in artificial salvia at 37° C in ultrasonic bath for different time period and then determined by U-HPLC, PDA detector. Three samples were prepared from each sample and three injections were made. Caffeic acid was investigate in the samples. The optimum waiting period is set at 5 hours, which is considered to be a possible period since the gum adheres to the tooth and is a hardly soluble substance. Considering the anticarcinogenic effect of caffeic acid, cytotoxic test was carried out on specimens which were kept in saliva for 24 hours acid. After incubation of the saliva extract that including 10, 20, 50 µM caffeic acid in liver cancerous cells 1, 3, 6 hours, cell viability was observed.
How to Cite
When the article is accepted for publication in the HSQ authors transfer all copyright in the article to the Holistence Academy Ar-Ge Yazılım Yayıncılık Eğitim Danışmanlık ve Organizasyon Ticaret Ltd. Şti.The authors reserve all proprietary right other than copyright, such as patent rights.
Everyone who is listed as an author in this article should have made a substantial, direct, intellectual contribution to the work and should take public responsibility for it.
This paper contains works that have not previously published or not under consideration for publication in other journals.