ELECTROCHEMICAL BIOSENSOR FOR BRCA1 GENE AND TAMOXIFEN INTERACTION


Abstract views: 12290 / PDF downloads: 221

Authors

DOI:

https://doi.org/10.26900/jsp.4.004

Keywords:

Tamoxifen, BRCA1, Electrochemical DNA nanobiosensor, carbon nanotube, Differential Pulse Voltammetry, Drug-DNA interaction, Pencil Graphite Electrode, Screen Printed Electrode

Abstract

The electrochemical nanobiosensor was designed for the determination of specific DNA sequences related to breast cancer 1 (BRCA1) gene and interaction between Anticancer Drug Tamoxifen (TAM) and related DNA sequences by using pencil graphite electrode (PGE), bare and multi-walled carbon nanotube (MWCNT) contained screen printed carbon electrodes (SPEs) for the first time. Here, biomolecular interaction between TAM and DNA was investigated differential pulse voltammetry (DPV) based on not only guanine signal but also TAM oxidation response. It was obtained that the guanine signal at about +1.00V obtained from probe DNA or hybrid DNA shows a remerkable increase after the interaction with TAM. Additionally, it was found that TAM interact with guanine bases and TAM signal which is near the guanine oxidation area also increase after the interaction with DNA. Consequently, the prepared biosensor offer suitable platform for the analysis of DNA hybridization and TAM-DNA interaction sensitively.

Downloads

Download data is not yet available.

References

BAGNI, G, MASCINI, M., 2010. DNA Biosensor for Environmental Risk Assessment and Drugs Studies. In: Sensors for Chemical and Biological Applications. Ed.: Ram, M. K., Bhethanabotla, V. R., Boca Raton, FL: CRC Press. 249–276.
DANESHGAR, P., NOROUZI, P., GANJALI, M. R., ZAMANI, H. A. 2009. Ultrasensitive flow-injection electrochemical method for detection of anticancer drug tamoxifen, Talanta, 77: 1075-1080.
HURTADO-MONROY, R., VARGAS-VIVEROS, P., CARRILLO-MUÑNOZ, S., DUENAS-GONZALEZ, A., 2007. Tamoxifen-associated vasculitis in a breast cancer patient, World Journal of Surgical Oncology, 5-7
KATZ, E., WILLNER, I., WANG, J., 2004. Electroanalytical and Bioelectroanalytical Systems Based on Metal and Semiconductor Nanoparticles, Electroanal., 16: 19-44.
KEİSHAM, R., PROBHAT, K., RAJU K., 2012. Detection of anticancer drug tamoxifen using biosensor based on polyaniline probe modified with horseradish peroxidase, Materials Science and Engineering C,33: 583-587.
KELSEY, J. L., BERNSTEIN, L., 1996. Epidemiology and Prevention of Breast Cancer, Annual Review of Public Health, 17:47-67.
OZKAN, D., KARADENIZ H., ERDEM A., MASCINI M., OZSOZ M., 2004. Electrochemical genosensor for Mitomycin C- DNA interaction based on guanine signal, Journal of Pharmaceutical and Biomedical Analysis, 35(4), 905-912
PATOLSKY, F., LIEBER, C.M., 2005. Nanowire nanosensors, Materials Today, 8(4): 20-28.
PUMERA, M., SANCHEZ, S., ICHINOSE, I., TANG, J., 2007. Electrochemical nanobiosensors, Sensors and Actuators B Chem., 123: 1195-1205.
SEYMA AYDINLIK, S., OZKAN-ARIKSOYSAL, D., KARA, P., SAYINER, A. A., OZSOZ, M., 2011. A nucleic acid-based electrochemical biosensor for the detection of influenza B virus from PCR samples using gold nanoparticle-adsorbed disposable graphite electrode and Meldola’s blue as an intercalator, Anal. Methods, 3, 1607–1614.
SUBAK, H., OZKAN-ARIKSOYSAL, D., 2018. Label-Free electrochemical biosensor for the detection of ınfluenza genes and the solution of guanine-based displaying problem of dna hybridization. Sens. Actuators B, 263, pp. 196-207
TOPKAYA, S. N., OZKAN-ARIKSOYSAL, D., 2016. Prostate cancer biomarker detection with carbon nanotubes modified screen printed electrodes, Electroanalysis, 28(5), 1077-1084.
WANG, J., 2005. Nanomaterial-based electrochemical biosensors, Analyst, 130: 421-426.
YARMAN, A., and SCHELLER. F. W., 2014. The first electrochemical mıp sensor for tamoxifen, Sensors,14, 7647-7654.

Downloads

Published

2020-01-31

How to Cite

YANIK, S., ÖZKAN-ARIKSOYSAL, D., & YILMAZ, S. (2020). ELECTROCHEMICAL BIOSENSOR FOR BRCA1 GENE AND TAMOXIFEN INTERACTION. HEALTH SCIENCES QUARTERLY, 4(1), 37–48. https://doi.org/10.26900/jsp.4.004

Issue

Vol. 4 No. 1 (2020)

Section

Letter to the Editor

License

When the  article is accepted for publication in the HSQ authors transfer all copyright in the article to the Holistence Academy Ar-Ge Yazılım Yayıncılık Eğitim Danışmanlık ve Organizasyon Ticaret Ltd. Şti.The authors reserve all proprietary right other than copyright, such as patent rights. 

Everyone who is listed as an author in this article should have made a substantial, direct, intellectual contribution to the work and should take public responsibility for it.

This paper contains works that have not previously published or not under consideration for publication in other journals.

Most read articles by the same author(s)