Effects of Plantaricin A on cytokine modulation and calcium homeostasis in Caco-2 cells treated with rotavirus NSP4 protein

Merve Şen; Hüseyin Taşlı; Sefa Çelik

doi:10.26900/hsq.3136

Authors

Merve Şen Afyonkarahisar Health Sciences University / Türkiye 0000-0001-6696-0889
Hüseyin Taşlı Ege University / Türkiye 0000-0003-0216-1740
Sefa Çelik Afyonkarahisar Health Sciences University / Türkiye 0000-0002-5187-378X

10.26900/hsq.3136

Abstract

Rotavirus represents a predominant etiological agent of acute gastroenteritis among neonates and young pediatric populations. Rotavirus predominantly targets enterocytes, leading to diarrhea as a consequence of intestinal cellular impairment induced by the rotavirus nonstructural protein 4 (Nsp4) and subsequent stimulation of the enteric nervous system. Nsp4 serves as the principal protein accountable for the alterations in calcium (Ca²⁺) homeostasis within cells infected by rotavirus. This study investigated the effects of plantaricin A (PlnA), a Lactobacillus plantarum bacteriocin, on intracellular and extracellular Ca²⁺ and anti-inflammatory and proinflammatory cytokine levels in the Caco-2 intestinal cell line treated with the Nsp4 protein, which causes diarrhea and damage to enterocytes through various mechanisms in rotavirus infections. Nsp4 increased intracellular and extracellular Ca²⁺ levels over time, and PlnA suppressed this effect when applied with Nsp4. Consequently, PlnA has the capacity to diminish cytosolic Ca²⁺, a critical element utilized by the virus for replication during the initial phases of infection. PlnA increased the gene expression and protein levels of proinflammatory cytokines. In a comparable manner, PlnA elevated the transcriptional activity and protein concentrations of the anti-inflammatory cytokine IL-10, indicating that PlnA may exert an anti-inflammatory influence. If supported by in vivo studies, PlnA could be a therapeutic agent for treating viral enteritis.

Keywords:

Bacteriocin Caco-2 cell line Lactobacillus plantarum Rotavirus Rotavirus nonstructural protein 4 Plantaricin A

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Effects of Plantaricin A on cytokine modulation and calcium homeostasis in Caco-2 cells treated with rotavirus NSP4 protein

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